The association of serum proteins with peripheral blood cells at low ionic strength: Ultrastructural examination

Summary Peripheral white blood cells have been shown to bind serum immunoglobins when prepared in low ionic strength sucrose solutions. The presence and distribution of Ig on neutrophils, monocytes and lymphocytes was described using an immunoferritin probe and the electron microscope. It appears that this approach reveals immunoglobulins associated with peripheral white blood cells that are not observed by the usual methods of examining these cell surfaces after preparation at physiological ionic strength.Supported by Grant Number AI 12750.Presented in part at Thirty-First Annual EMSA Meeting 1974, and again in part at the Seventy Sixth Annual. American Society of Microbiology, 1976.Predoctoral supported on Training Grant No. 1-Tol-CA-052595.