Genetic diversity in some grape varieties revealed by SCoT analyses |
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Authors: | Da-Long Guo Jun-Yu Zhang Chong-Huai Liu |
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Institution: | College of Forestry, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China. guodalong@mail.haust.edu.cn |
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Abstract: | Start codon targeted (SCoT) polymorphic markers were used to assess genetic relationships among 64 grape varieties. Seventeen
informative primers were selected from 36 SCoT primers based on their ability to produce clear and repeatable polymorphic
and unambiguous bands among the varieties. A total of 131 bands were produced; 93.1% of them were polymorphic; the average
polymorphism information content was 0.82. Cluster analysis of SCoT markers through the unweighted pair-group method of arithmetic
averages analysis and principal coordinate analysis were largely consistent. The partition of clusters in the dendrogram and
PCoA plot was similar and some degree of grouping by types of grape and taxonomic status of the varieties was revealed. Four
main groups were found after cluster analysis, i.e. table grape of Vitis vinifera; table grape of Euro-America hybrid; wine grape of V. vinifera and wild Vitis species. The results showed that the wild Vitis species originated from America and China could be clearly differentiated. The results also indicated that SCoT markers are
informative and could be used to detect polymorphism for grape varieties. |
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