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The application of a high throughput analysis method for the screening of potential biosurfactants from natural sources |
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| Authors: | Chen Chien-Yen Baker Simon C Darton Richard C |
| Institution: | aDepartment of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, UK bDepartment of Earth and Environmental Sciences, National Chung Cheng University, 168 University Road, Min-hsiung, Chia-yi 621, Taiwan cSchool of Life Sciences, Oxford Brookes University, Gypsy Lane, Oxford OX3 0BP, UK |
| Abstract: | The presence of biosurfactants in growth media can be evaluated by a variety of methods, none of which are suitable for high throughput studies. The method described here is based on the effect of meniscus shape on the image of a grid viewed through the wells of a 96-well plate. The efficacy of the method was demonstrated by the selection of a bacterium (producing a biosurfactant able to reduce the surface tension of pure water from 72 to 28.75 mN m− 1) from a culture collection isolated from aviation fuel-contaminated land. The assay was found to be more sensitive, rapid and easy to perform than other published methods. It does not need specialised equipment or chemicals and excludes the bias which results from the surfactant properties of medium used for bacterial growth. |
| Keywords: | Biosurfactant Emulsification test Surface tension High throughput screening |
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