Putative roles of bacteria in the carbon and nitrogen cycles in a tropical peat swamp forest |
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| Institution: | 1. School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Subang Jaya 47500, Malaysia;2. Department of Biochemistry and Microbiology, Faculty of Sciences, University of Ghent, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium;3. Tropical Medicine & Biology Multidisciplinary Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Subang Jaya 47500, Malaysia;4. School of Science, Technology and Engineering, University of the Sunshine Coast, Sippy Downs, Queensland 4556, Australia;5. Department of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany;6. Center for Computational and Theoretical Biology, University of Würzburg, Am Hubland, 97074 Würzburg, Germany;1. Department of Geography, McGill University, 805 Sherbrooke Street West, Montreal, Quebec H3A OB9, Canada;2. Institute for Landscape Ecology, Ecohydrology and Biogeochemistry Group, Heisenbergstr. 2, University of Münster, 48149 Münster, Germany;3. Department of Environmental Studies, Mount Holyoke College, 50 College Street, South Hadley, MA 01075, USA;1. Center for Southeast Asian Studies, Kyoto University, Kyoto 606-8501, Japan;2. Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan;3. CIMTROP, University of Palangkaraya, Palangkaraya, 73112, Indonesia |
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| Abstract: | Tropical peat swamp forests are waterlogged, acidic, anoxic and oligotrophic ecosystems. They are important terrestrial carbon pools that help mitigating global warming through carbon sequestration in peat. This study aimed at investigating putative roles of bacteria in the carbon and nitrogen cycles in North Selangor peat swamp forest, Malaysia. Whole-genome sequencing was performed on four bacterial isolates using Illumina NextSeq 500 to decipher their genetic information while Gen III Microplate (Biolog) was applied to verify carbon source utilization. The isolates were identified as Dyella sp. strain C9, Dyella sp. strain C11, Klebsiella sp. strain C31 and Paraburkholderia sp. strain C35. Both Dyella spp. and Paraburkholderia sp. strain C35 were likely novel species while Klebsiella sp. strain C31 was a different strain of the type species, Klebsiella pneumoniae. Both genomic and bioassay results suggested the involvement of the isolates in the degradation of lignocellulose, carbohydrates, sugar alcohols, organic acids and aromatic compounds. The isolates could potentially perform methanotrophy, which helps to mitigate methane emissions from tropical peatlands. In addition, the isolates also contained genes encoding enzymes for nitrite, nitrate and nitric oxide reduction, as well as dissimilatory nitrate reduction to ammonium, which retains the nitrogen in the ecosystems. The results generated insights into potential functions of bacteria in the energy production and nutrient cycling of tropical peatlands, which are essential for the sustainability of high biomass and biodiversity in these ecosystems. |
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