Proton pump coupled to cytochrome c oxidase in Paracoccus denitrificans |
| |
Authors: | HW Van Verseveld K Krab AH Stouthamer |
| |
Institution: | Department of Microbiology, Biological Laboratory, Free University, De Boelelaan 1087, 1007 MC Amsterdam The Netherlands |
| |
Abstract: | The proton translocating properties of cytochrome c oxidase in whole cells of Paracoccus denitrificans have been studied with the oxidant pulse method. quotients have been measured with endogenous substrates, added methanol and added ascorbate (+TMPD) as reductants, and oxygen and ferricyanide as oxidants. It was found that both the observed with ascorbate (+TMPD) as reductant, and the differences in proton ejection between oxygenand ferricyanide pulses, with endogenous substrates or added methanol as a substrte, indicate that the P. denitrificans cytochrome c oxidase translocates protons with a stoichiometry of . The results presented in this and previous papers are in good agreement with recent findings concerning the mitochondrial cytochrome c oxidase, and suggest unequal charge separation by different coupling segments of the respiratory chain of P. denitrificans. |
| |
Keywords: | Methanol Respiratory chain Proton translocation Ascorbate (Paracoccus denitrificans) |
本文献已被 ScienceDirect 等数据库收录! |
|