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Phospholipid chain immobilization and steriod rotational immobilization in acetylcholine receptor-rich membranes from torpedo marmorata
Authors:D Marsh  A Watts  F.J Barrantes
Affiliation:1. Max-Planck-Institut für biophysikalische Chemie, D-3400 Göttingen F.R.G.
Abstract:1. The ESR spectra of both phosphatidylcholine and phosphatidylethanolamine spin labels reveal an immobilized lipid component (τR ? 50 ns), in addition to a fluid component (τR ~ 1 ns), in acetylcholine receptorrich membranes prepared from Torpedo marmorata electroplax according to the method of Cohen et al. (Cohen, J.B., Weber, M., Huchet, M. and Changeux, J.P. (1972) FEBS Lett. 26, 43–47). 2. The ESR spectra of the androstanol spin label display a component corresponding to molecules which are immobilized with respect to rotation about the long molecular axis (
/></figure>), in addition to the fluid lipid bilayer component in which the molecules are rotating rapidly about their long axes (<figure class=/></figure>). This immobilized component is observed throughout the temperature range 2–22°C, at an approximately constant relative intensity of approx. 45% of the total, which is quantitatively the same as previously observed with fatty acid spin labels.</td>
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Keywords:Spin label  Phospholipid chain  Steroid  Rotational immobilization  Acetylcholine receptor  (Torpedo marmorata membrane)  ASL, 17-β-hydroxy-4′,4′-dimethylspiro[5α-androstane-3,2′-oxazolidin]-3′-yloxyl
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