Construction of integrated system for selection of fused plant protoplasts |
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| Authors: | Kazuo Sakamoto Kazuhiro Fujita Kimiko Hirayae Kumiko Iida Takashi Koyano Yoshihisa Asada Tsutomu Furuya |
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| Affiliation: | (1) Saitama Laboratory, P.C.C. Technology Inc., c/o Tonen Corporation, 1-3-1 Nishi-tsurugaoka, Ohimachi, Iruma-gun, 356 Saitama, Japan;(2) J.S.I. Laboratory, Japan Scientific Instrument Co., Ltd., 6-7-5, Higashi-kasai, Edogawa-ku, 134 Tokyo, Japan;(3) School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, 108 Tokyo, Japan |
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| Abstract: | Summary An integrated system has been constructed to instantly identify and efficiently sort the heterokaryons formed by plant protoplast fusion. The system is composed of the following functions: a) a transport system, b) an electro-manipulator, c) a cell harvester, d) a flow cytometer/cell sorter, and e) a control device. The conditions for an efficient and reproducible enrichment of the heterokaryons have been investigated by this system using the fluorescein isothiocyanate stained protoplasts preparing from Glycyrrhiza glabra cell cultures and unstained protoplasts of Abrus precatorius cell cultures which contain a large quantity of chlorophyll.Abbreviations MS Murashige and Skoog (1962) - 2,4-D 2, 4-dichlorophenoxyacetic acid - NAA 1-naphthaleneacetic acid - BAP 6-benzylaminopurine - ABA abscissic acid - FITC fluorescein isothiocyanateThis paper is part 96 in the series  Studies on Plant Tissue Cultures . For part 95 see Orinara Y., Noguchi T. and Furuya T. (1993) submitted for publication. |
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