| LTSE—新的大肠菌群快速检测法 |
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| 引用本文： | 涂楚国.LTSE—新的大肠菌群快速检测法[J].中国微生态学杂志,1990,2(4):57-61. |
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| 作者姓名： | 涂楚国 |
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| 作者单位： | 湖南省湘潭市卫生防疫站 湘潭411100 |
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| 摘 要： | 本文报道快速简易、结果准确、应用广泛、效益明显,总大肠菌群和粪大肠菌群在15～18小时内同时检出,费用比部颁三步发酵法(GB)节省75～84%的大肠菌群检测新方法。笔者研究出 Lactose-Tryptone-Sodium Dodecyl sulfate-Trace Elements简称 LTSE]培养基,加上几种鉴定验证试验的 LTSE 法,用来快速检测水质、食品、冷饮、餐具等各类样品703件,结果与国标法(GB)对照总符合率为99.3%;同时与美国《水和废水标准检验法》(16版1985年)新发展的现行标准检测粪大肠菌群的正式 A-1法相比较特异性强,灵敏度高,能提高检出率,并节约经费38.1%.
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| 关 键 词： | LTSE 培基 A-1 GB 氧化酶 吲哚 |
A New Rapid and Economic Method for Detecting Coliform Bacilli |
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| Tu Chu-guo Xiangtan Sanitary and Anti-epidemic Station,Xiangtan ,Hunan Province.A New Rapid and Economic Method for Detecting Coliform Bacilli[J].Chinese Journal of Microecology,1990,2(4):57-61. |
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| Authors: | Tu Chu-guo Xiangtan Sanitary and Anti-epidemic Station Xiangtan Hunan Province |
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| Institution: | Tu Chu-guo Xiangtan Sanitary and Anti-epidemic Station,Xiangtan 411100,Hunan Province |
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| Abstract: | A simple,rapid and reproducible method for detecting coliform bacilli was developed. The Lactose-Tryptone-Sodium Dodecyl Sulfate- Trace Elements medium LTSE medium]was designed and used as the basic medium for LTSE method.The growth of coliform bacilli was selectively enhanced by LTSE medium,and growth of other bacteria was in- hibited.Combining the LTSE method with conforming tests,the results could be reported in 15 or 18.hours.703 samples taken from water,food,cold beverage and so on were tes- ted with A-1 method of America,GB method issued by Ministry of Public Health of China as well as the LTSE method.Results indica- ted that LTSE method is more specific,sen- sitive,rapid and economic. |
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| Keywords: | Lactose-Tryptone-Sodium Dodecyl Sulfate-Trace Elements medium A-l GB coliform bacilli Oxidase Indole |
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