蒜头果内生真菌次生代谢产物抑制人类致病菌活性的研究

蒜头果是我国特有的单种属稀有树种,为了进一步开发利用蒜头果树皮内生真菌的抗菌活性化合物,该研究对来自蒜头果的植物内生真菌(白黄笋顶孢霉、哈茨木霉、大棘黑团孢、枝状枝孢菌、斑污拟盘多毛孢、赭绿青霉、淡紫紫孢菌、朱黄青霉、Xenoacremonium recifei、Xylaria feejeensis)进行液体培养,10 d后回收培养液并用乙酸乙酯萃取获得初提物,采用抑菌圈法检测蒜头果内生真菌初提物抑菌活性,同时测定了最低抑菌浓度(MIC)。结果表明:白黄笋顶孢霉、大棘黑团孢、枝状枝孢菌、斑污拟盘多毛孢、赭绿青霉、淡紫紫孢菌均有抑菌活性,大棘黑团孢、斑污拟盘多毛孢、淡紫紫孢菌的初提物均对缓慢芽孢杆菌、无乳链球菌和藤黄微球菌有明显抗菌活性,最低抑菌浓度在1.562 5~6.25 mg·m L~(-1)之间。这说明蒜头果树皮内生真菌的次生代谢产物具有抗菌活性,各内生真菌次生代谢产物的抗菌效果不同。

Activity inhibition of human pathogenic bacteria by secondary metabolites from Malania oleifera endophytic fungi

Malania oleifera is an endemic and rare species in China. In order to explore and utilize the antibacterial active compounds of endophytic fungi from M. oleifera bark, endophytic fungi(Acrostalagmus luteoalbus, Trichoderma harzianum,Periconia macrospinosa,Cladosporium cladosporioides,Pestalotiopsis maculans,Penicillium ochrochloron, Purpureocillium lilacinum, Penicillium minioluteum, Xenoacremonium recifei and Xylaria feejeensis)from Malania oleifera were cultured in liquid media. After 10 d culture, the culture media were collected, and then the crude extracts were obtained with ethyl acetate extraction. The inoculating inhibition zone was used to evaluate the antibacterial activity of the crude extracts. Meanwhile, the minimum inhibitory concentration(MIC)of antibacterial activity was also detected. The results showed that the crude extracts of Acrostalagmus luteoalbus, Periconia macrospinosa, Cladosporium cladosporioides,Pestalotiopsis maculans, Penicillium ochrochloron and Purpureocillium lilacinum had antibacterial activities, the crude extracts of Periconia macrospinosa, Pestalotiopsis maculans and Purpureocillium lilacinum had significant antibacterial activities against Bacillus lentus, Streptcococcus agalactiae and Micrococcus luteus. The minimum inhibitory concentration was between 1.562 5 and 6.25 mg·mL^-1. This results showed that secondary metabolites of endophytic fungi of Malania oleifera bark had antibacterial activities and the antibacterial effects of secondary metabolites of endophytic fungi were different.