Isolation and characterization of chitinase from a marine bacterium Vibrio sp. |
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| Authors: | Zhou S.N. Yang C.Y. Lu Y.J. Huang L. Cai C.H. Lin Y.C. |
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| Affiliation: | (1) State Key Laboratory for Biocontrol, Department of Biochemistry, Zhongshan University, Guangzhou, P.R. China;(2) South China Sea, Institute of Oceanology, Academia Sinica, P.R. China |
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| Abstract: | A chitinase was separated from the culture broth of Vibrio sp. 11211 isolated from sediment from the South China Sea. The chitinase was purified 18.3-fold with 33% recovery by ammonium sulphate precipitation and chromatography. The subunit molecular weight of the enzyme was estimated by SDS-PAGE to be about 30 kDa. The enzyme showed optimum pH at 6.5 and optimum temperature at 50°C, and was stable in the pH range of 4 to 9 and at the temperature below 40°C. |
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| Keywords: | Chitinase marine bacteria Vibrio sp. |
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