| 复合菌系RSS-4传代对水稻秸秆腐解的影响 |
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| 引用本文： | 刘甲锋,沈德龙,李力,陈慧君,关大伟,姜昕,李俊.复合菌系RSS-4传代对水稻秸秆腐解的影响[J].微生物学杂志,2010,30(4):30-35. |
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| 作者姓名： | 刘甲锋 沈德龙 李力 陈慧君 关大伟 姜昕 李俊 |
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| 作者单位： | 中国农业科学院,农业资源与农业区划研究所,北京,100081 |
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| 基金项目： | 国家十一五科技支撑项目 |
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| 摘 要： | 对筛选到的1组稻秆腐解复合菌系RSS-4,以腐解产物的pH值、酶活及稻秆失重率为指标,研究了该复合菌系的性质稳定性和功能稳定性;并用变性梯度凝胶电泳法(DGGE)分析了该复合菌系的菌种组成稳定性。结果表明,多代继代培养过程中各代复合菌系腐解产物的pH值变化趋势基本一致,pH值从起始的6.72左右迅速升至最高点7.75,然后逐渐下降并稳定在7.20;多代继代培养后复合菌系各代之间的酶活动态变化及其对稻秆腐解能力的差异均很小。在连续继代培养的过程中,DGGE图谱揭示第15~23代复合菌系之间的菌种组成没有明显差异。
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| 关 键 词： | 复合菌系 稳定性 变性梯度凝胶电泳 酶活 |
Effects of Generation Passing of Composite Microbial System RSS-4 on Rice Straw Decomposition |
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| LIU Jia-feng,SHEN De-long,LI Li,CHEN Hui-jun,GUAN Da-wei,JIANG Xin,LI Jun.Effects of Generation Passing of Composite Microbial System RSS-4 on Rice Straw Decomposition[J].Journal of Microbiology,2010,30(4):30-35. |
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| Authors: | LIU Jia-feng SHEN De-long LI Li CHEN Hui-jun GUAN Da-wei JIANG Xin LI Jun |
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| Institution: | LIU Jia-feng,SHEN De-long,LI Li,CHEN Hui-jun,GUAN Da-wei,JIANG Xin,LI Jun(Inst.of Agric.Res.& Reg.Planning,Chinese Acad.of Agric.Sci.,Beijing 100081) |
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| Abstract: | A rice straw-degradation Complex Microbial System RSS-4 was screened and its decomposition function property and stability were studied by evaluating the pH value,CMC activity,FPA activity,xylan activity and weight-losing rate of the microorganism-treated rice straw.The stability of microorganism composition was studied by denaturing gradient gel electrophoresis(DGGE) technique.It was found that in the continuous subculture,the pH value of the system had a similar changing law in each generation,i.e.,increa... |
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| Keywords: | complex microbial system stability denaturing gradient gel electrophoresis(DGGE) enzyme activity |
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