| 外源性蛋白酶对肉毒毒素的作用初探 |
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| 引用本文： | 张雪平,何星,李小娟,王云天. 外源性蛋白酶对肉毒毒素的作用初探[J]. 微生物学免疫学进展, 2009, 37(3): 4-7 |
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| 作者姓名： | 张雪平 何星 李小娟 王云天 |
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| 作者单位： | 兰州生物制品研究所,兰州,730046 |
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| 摘 要： | 将B型肉毒毒素在毒素粗提阶段用胰蛋白酶处理,再经浓缩、柱层析和结晶得到纯化的B型肉毒毒素复合物。结果表明:B型肉毒神经毒素经胰蛋白酶处理后单链裂解为双链,在非还原条件下SDS-PAGE显示神经毒素条带,在还原条件下SDS-PAGE只显示轻(L)、重(H)二链条带,而不显示神经毒素条带;纯化后毒素复合物的比活性提高了5.9倍,达到1.60×108LD50/mgPr;HPLC显示活性成分峰面积所占比例增加了9.83%。
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| 关 键 词： | 肉毒梭菌 B型肉毒毒素 胰蛋白酶 |
Preliminary study on the effect of exogenous protease on Botulinum toxin |
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| Abstract: | Botulinum toxin type B was activated with trypsin during extraction,the purified toxin complex was prepared by concentration,chromatography and crystallization.The results showed that after activating with trypsin,the single-chain of botulinum neurotoxin type B molecule is cleaved into the di-chain form;SDS-PAGE show neurotoxin band,but in reduced conditions the band disappear;The specific toxicity of toxin complex is 1.60×108 LD50/mgPr,increasing 5.9 times;profile of HPLC show active peak area increasing 9.83%. |
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| Keywords: | Clostridium botulinum Botulinum toxin type B Trypsin |
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