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Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose |
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| Authors: | M R Smith C S Devine S M Cohn M W Lieberman |
| Affiliation: | Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110 USA |
| Abstract: | A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp. |
| Keywords: | agarose gel electrophoresis DNA electrophoretic transfer hybridization nitrocellulose polyacrylamide gel electrophoresis |
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