Use of DNA marker to select well-adapted <Emphasis Type="Italic">Phaseolus</Emphasis>-symbionts strains under acid conditions and high temperature |
| |
Authors: | Abdelaal Shamseldin |
| |
Institution: | (1) Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technological Applications, P.O. Box 21934, New Borg El-Arab City, Alexandria, Egypt |
| |
Abstract: | Soil acidity and high temperature contribute to the failure of nodulation in the common bean. It is therefore urgent to select
strains with a high competitive ability under these stress conditions. Two Egyptian Rhizobium etli strains, EBRI 2 and EBRI 26, were examined against Rhizobium tropici CIAT 899G labeled with the gus (β-glucuronidase) reporter gene. EBRI 2 and EBRI 26 were less competitive than CIAT 899G under acid conditions with both
the Egyptian cultivar Giza 3 and the Colombian cultivar Rab 39. However, EBRI 2 and EBRI 26 gave higher nodule occupancy (78%
and 62.5, respectively) than the nodule occupancy (18.5% and 35%) obtained by CIAT 899G at 35°C with cultivar Giza 3. Soil
acidity (pH 5.8) was less detrimental to the nodule occupancy of EBRI 2 than EBRI 26 when they tested in competition with
CIAT 899G. |
| |
Keywords: | DNA marker Phaseolus Rhizobium competition Soil acidity |
本文献已被 PubMed SpringerLink 等数据库收录! |
|