Identification of New Interactions between Endolysosomal Tethering Factors |
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| Affiliation: | 1. Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary;2. Premium Postdoctoral Research Program, Eötvös Loránd Research Network, Budapest, Hungary;3. Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary;4. Institute of Genetics, Biological Research Centre, Szeged, Hungary |
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| Abstract: | Proper functioning of the precisely controlled endolysosomal system is essential for maintaining the homeostasis of the entire cell. Tethering factors play pivotal roles in mediating the fusion of different transport vesicles, such as endosomes or autophagosomes with each other or with lysosomes. In this work, we uncover several new interactions between the endolysosomal tethering factors Rabenosyn-5 (Rbsn) and the HOPS and CORVET complexes. We find that Rbsn binds to the HOPS/CORVET complexes mainly via their shared subunit Vps18 and we mapped this interaction to the 773–854 region of Vps18. Based on genetic rescue experiments, the binding between Rbsn and Vps18 is required for endosomal transport and is dispensable for autophagy. Moreover, Vps18 seems to be important for β1 integrin recycling by binding to Rbsn and its known partner Vps45. |
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| Keywords: | Rabenosyn-5 HOPS CORVET Vps18 Vps45 Rbsn" },{" #name" :" keyword" ," $" :{" id" :" k0035" }," $$" :[{" #name" :" text" ," _" :" Rabenosyn-5 Vps" },{" #name" :" keyword" ," $" :{" id" :" k0045" }," $$" :[{" #name" :" text" ," _" :" Vacuolar Protein Sorting Rab" },{" #name" :" keyword" ," $" :{" id" :" k0055" }," $$" :[{" #name" :" text" ," _" :" Ras-Associated Binding Arf" },{" #name" :" keyword" ," $" :{" id" :" k0065" }," $$" :[{" #name" :" text" ," _" :" ADP-Ribosylation Factor SNARE" },{" #name" :" keyword" ," $" :{" id" :" k0075" }," $$" :[{" #name" :" text" ," _" :" SNAP Receptor, (SNAP: Soluble NSF Attachment Protein) SM protein" },{" #name" :" keyword" ," $" :{" id" :" k0085" }," $$" :[{" #name" :" text" ," _" :" Sec1/Munc-18 protein EEA1" },{" #name" :" keyword" ," $" :{" id" :" k0095" }," $$" :[{" #name" :" text" ," _" :" Early Endosomal Antigen 1 CORVET" },{" #name" :" keyword" ," $" :{" id" :" k0105" }," $$" :[{" #name" :" text" ," _" :" Class-C Core Vacuole/Endosome Tethering HOPS" },{" #name" :" keyword" ," $" :{" id" :" k0115" }," $$" :[{" #name" :" text" ," _" :" Homotypic Fusion and Vacuole Protein Sorting GARP" },{" #name" :" keyword" ," $" :{" id" :" k0125" }," $$" :[{" #name" :" text" ," _" :" Golgi-Associated Retrograde Protein EARP" },{" #name" :" keyword" ," $" :{" id" :" k0135" }," $$" :[{" #name" :" text" ," _" :" Endosome-Associated Recycling Protein CHEVI" },{" #name" :" keyword" ," $" :{" id" :" k0145" }," $$" :[{" #name" :" text" ," _" :" Class-C Homologs in Endosome–Vesicle Interaction FERARI" },{" #name" :" keyword" ," $" :{" id" :" k0155" }," $$" :[{" #name" :" text" ," _" :" Factors for Endosome Recycling and Retromer Interaction EHD1" },{" #name" :" keyword" ," $" :{" id" :" k0165" }," $$" :[{" #name" :" text" ," _" :" EH Domain Containing Protein 1 ANK3" },{" #name" :" keyword" ," $" :{" id" :" k0175" }," $$" :[{" #name" :" text" ," _" :" Ankyrin-3 PI3P" },{" #name" :" keyword" ," $" :{" id" :" k0185" }," $$" :[{" #name" :" text" ," _" :" Phosphatidylinositol-3-Phosphate LC3" },{" #name" :" keyword" ," $" :{" id" :" k0195" }," $$" :[{" #name" :" text" ," _" :" Microtubule-Associated Proteins 1A/1B Light Chain 3B HEK293" },{" #name" :" keyword" ," $" :{" id" :" k0205" }," $$" :[{" #name" :" text" ," _" :" Human Embryonic Kidney 293 Y2H" },{" #name" :" keyword" ," $" :{" id" :" k0215" }," $$" :[{" #name" :" text" ," _" :" yeast two-hybrid method |
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