Different acute in vivo effects of bacterial derived and pituitary growth hormone preparations on plasma levels of glucagon, insulin and free fatty acids in rabbits

Intravenous injection of high doses of bacterial derived growth hormone (1 and 2 mg Somatonorm) did not affect the plasma levels of glucagon, insulin and free fatty acids in fasted and fed rabbits. On the other hand, 1 and 2 mg human extracted growth hormone (Crescormon) had stimulatory effects on plasma levels of glucagon, insulin and free fatty acids. These results indicate that the observed stimulatory effects in the rabbits were not due to the growth hormone molecule itself. It was shown by gel filtration and SDS electrophoresis that Crescormon contained constituents that were not present in Somatonorm. The differences in the observed metabolic effects of bacterial derived and pituitary growth hormone preparations in the rabbits could possibly be attributed to a human pituitary lipid-mobilizing factor LMF.