Cleavage of nucleotides by Ce4+ and the lanthanide metal complexes |
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| Authors: | Bing Zhu Yi-Jie Wu Da-Qing Zhao Jia-Zuan Ni |
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| Affiliation: | (1) Department of BioInorganic Chemistry, School of Pharmaceutical Science, Beijing Medical University, Beijing, 100083, China;(2) Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P.R. China |
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| Abstract: | The cleavage of adenosine-5 -monophosphate (5-AMP) and guanosine-5-monophosphate (5-GMP) by Ce4+ and lanthanide complex of 2-carboxyethylgermanium sesquioxide (Ge-132) in acidic and near neutral conditions was investigated by NMR , HPLC and measuring the liberated inorganic phosphate at 37°C and 50°C. The results showed that 5-GMP and 5-AMP was converted to guanine (G), 5-monophosphate (depurination of 5-GMP), ribose (depurination and dephosphorylation of 5-GMP), phosphate and adenine (A), 5-monophosphate (depurination of 5-AMP), ribose (depurination and dephosphorylation of 5-AMP), phosphate respectively by Ce4+. In presence of lanthanide complexes, 5-GMP and 5-AMP were converted to guanosine (Guo) and phosphate and adenosine (Ado) and phosphate respectively. The mechanism of cleaving 5-GMP and 5-AMP is hydrolytic scission |
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| Keywords: | adenosine-5![]("</a) /content/m2873x47225t130x/xxlarge8242.gif" alt=" prime" align=" BASELINE" BORDER=" 0" >-monophosphate and guanosine-5 /content/m2873x47225t130x/xxlarge8242.gif" alt=" prime" align=" BASELINE" BORDER=" 0" >-monophosphate cleavage Ce(NH4)2(NO3)6 and Ce(SO4)2 lanthanide ions lanthanide complexes |
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