Properties of thermolysin immobilized in polymer matrix by radiation polymerization |
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| Authors: | Minoru Kumakura Isao Kaetsu Takeshi Kobayashi |
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| Affiliation: | Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute, Takasaki, Gunma, Japan;Department of Chemical Engineering, Nagoya University, Nagoya, Japan |
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| Abstract: | Thermolysin (Bacillus thermoproteolyticus neutral proteinase, EC 3.4.24.4) has been immobilized by radiation polymerization of hydrophilic and hydrophobic monomers, and its properties, such as enzyme activity, thermal stability and durability, have been studied. The activity of the immobilized enzymes increased with an increase in the hydrophilicity of the polymer matrix and with a decrease in monomer concentration. Immobilization with hydrophilic monomers increased the thermal stability of the enzymes, but the thermal stability of the enzymes immobilized with hydrophobic monomers was comparable with that of native enzymes. The durability of the immobilized enzymes was examined by continuous hydrolysis of casein; enzymes immobilized with a high concentration (90%) of hydrophilic monomers appeared to be stabilized and could be used for long times. |
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| Keywords: | Proteins thermolysin immobilization radiation polymerization enzyme activity thermal stability hydrophilicity |
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