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Enzyme immobilization on grafted polymeric microspheres |
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| Authors: | Douglas S. Clark James E. Bailey Richard Yen Alan Rembaum |
| Affiliation: | Department of Chemical Engineering, California Insitute of Technology, Pasadena, California 91125, USA;Jet Propulsion Laboratory, Pasadena, California 91109, USA |
| Abstract: | Radiation-mediated grafting of polyacrolein onto poly(methyl methacrylate) microspheres has been shown to activate the particles for chymotrypsin (EC 3.4.21.1) immobilization. Treatment of porous polystyrene/magnetite particles with polyacrolein produced very small enzyme loading enhancement and significantly increased substrate diffusional resistance. |
| Keywords: | Polymers immobilized chymotrypsin enzyme supports polymer radiation grafting magnetic carriers |
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