Use of a bioelectrochemical cell for the synthesis of (bio)chemicals |
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| Authors: | Colja Laane Wouter Pronk Maurice Franssen Cees Veeger |
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| Affiliation: | 1. Department of Biochemistry, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands;2. Department of Organic Chemistry, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands |
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| Abstract: | ![]()
A bioelectrochemical cell containing either d-glucose oxidase (β-d-glucose:oxygen 1-oxidoreductase, EC 1.1.3.4) or xanthine oxidase (xanthine:oxygen oxidoreductase, EC 1.2.3.2) plus dichlorophenol-indophenol as electron acceptor in one half-cell, and chloroperoxidase (chloride:hydrogen-peroxide oxidoreductase, EC 1.11.1.10) in the other half-cell is described. Due to a combination of chemical, biochemical and electrochemical reactions, electricity and specific (bio)chemicals can be produced in the cell simultaneously and in both compartments. Furthermore, the oxidases in a bioelectrochemical cell are not inactivated by H2O2 and as a result the operational lifetimes of the oxidases were increased about five-fold. |
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| Keywords: | Enzyme stabilization bioelectrochemical cell glucose oxidase xanthine oxidase synthesis of biochemicals |
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