Micropropagation of Uniola paniculata L. |
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Authors: | John C Hovanesian Kenneth C Torres |
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Institution: | (1) Department of Horticulture, 137 Julian C. Miller Hall, Louisiana State University Agricultural Center, 70806 Baton Rouge, LA, USA |
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Abstract: | Uniola paniculata L. is a major sand-dune stabilizing grass which is being utilized to prevent shoreline erosion. In vitro cultured caryopses of U. paniculata produced callus on MS medium supplemented with 22.5 M 2,4-D, 4.4 M BA and 87.6 mM sucrose. Shoot induction occurred after these calli were inoculated onto the same medium without 2,4-D. Rooting of in vitro-derived shoots occurred when transferred to a one-half strength MS medium containing 43.8 mM sucrose and 14.7 M IBA. Plantlets were planted after the roots reached a length greater than 20 mm.Abbreviations BA
N-(phenyl-methyl)-lH-purine-6-amine
- IBA
lH-indole-3-butanoic acid
- MS
Murashige and Skoog (1962)
- 2,4-D
(2,4-dichlorophenoxy) acetic acid
- TC agar
tissue culture agar, (K.C. Biologicals, Lenexa, KS)
- Subdue
methaxyl:N-(2,6-dimethylphenyl)-N-(methyoxyacetyl) alanine methyl ester (CIBA- GEIGY) |
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Keywords: | |
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