| 产小檗碱内生真菌的诱变 |
| |
| 引用本文： | 高杨,殷红,孙宇宏,张志强,崔迎.产小檗碱内生真菌的诱变[J].菌物研究,2008,6(4):216-219. |
| |
| 作者姓名： | 高杨 殷红 孙宇宏 张志强 崔迎 |
| |
| 作者单位： | 西北大学生命科学学院,西安,710069 |
| |
| 基金项目： | 陕西省自然科学基础研究计划项目
，
陕西省中医管理局中医药科研项目
|
| |
| 摘 要： | 以产小檗碱的内生真菌S6为出发菌株,采用多种单一或复合诱变措施对其菌丝体进行诱变处理,最终筛选得到高产菌株S-NU-302。其小檗碱产量比出发菌株提高170%,达到12.28 mg/L;生长速率提高81.7%,达到5.72 g/L;经10次传代显示该菌株具有良好的遗传稳定性。
|
| 关 键 词： | 内生真菌 小檗碱 诱变 |
Mutagenesis of a Berberine-Producing Endophytic Fungus |
| |
| GAO Yang,YIN Hong,SUN Yu-hong,ZHANG Zhi-qiang,CUI Ying.Mutagenesis of a Berberine-Producing Endophytic Fungus[J].Journal of Fungal Research,2008,6(4):216-219. |
| |
| Authors: | GAO Yang YIN Hong SUN Yu-hong ZHANG Zhi-qiang CUI Ying |
| |
| Institution: | (College of Life Science, Northwest University, Xi＇an 710069, China) |
| |
| Abstract: | An initial berberine-producing endophytic fungus S6 was mutagenized by some single and multiple mutagenization.A dominant mutant strain(S-NU-302) was screened out.Its biomass increased by 81.7% compared with the initial strain,which rose up to 5.72 g/L.Berberine yield was effectively improved to 12.28 mg/L and was 170% higher than the initial strain.The yield of the product showed no distinct changes after successive culture of 10 generations. |
| |
| Keywords: | endophytic fungi berberine mutation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
