寒兰的快速繁殖技术

以寒兰(CymbidiumkanranMakino)根状茎为外植体,采用B5基本培养基,并附加不同浓度的6-BA、NAA、TDZ(苯基噻二唑基脲-thidiazuron)和S-3307(优康唑-uniconazole),对类原球茎的诱导、继代增殖、分化、生根等进行研究。结果表明:诱导类原球茎的最佳培养基为B5 TDZ0.50mgL-1 NAA0.25mgL-1,诱导率98.3%;继代增殖的最佳培养基为B5 S-33071.0mgL-1 NAA0.2mgL-1 蔗糖3.5%,增殖系数9.4;类原球茎分化的最佳培养基为B5 S-33070.75mgL-1 6-BA1.0mgL-1 NAA0.4mgL-1,分化率87.8%;最佳的生根培养基为1/2B5 NAA0.2mgL-1 活性炭0.05%,生根率达100%。

Rapid Propagation of Cymbidium kanran Makino

Propagation of Cymbidium kanran Makino by using rhizomes as explants to study the effects of growth regulators on the induction,multiplication and differentiation of protocorm-like bodies(PLBs),and the rooting.The results showed that the optimal medium for induction of PLBs was B5 containing 0.50 mg L-1 TDZ and 0.25 mg L-1 NAA,in which induction rate of PLBs was 98.3%,for multiplication of PLBs it was B5 containing 1.0 mg L-1 uniconazole(S-3307) and 0.2 mg L-1 NAA and 3.5% sucrose,the multiplication coefficient being 9.4.For differentiation it was best on B5 medium containing 0.75 mg L-1 S-3307,1.0 mg L-1 6-BA and 0.4 mg L-1 NAA,in which the differentiation rate was 87.8%.As for rooting,1/2B5 supplemented with 0.2 mg L-1 NAA and 0.05% active carbon,in which rooting rate reached 100%.