A new approach for rapidly reshaping single-chain antibody in vitro by combining DNA shuffling with ribosome display |
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Authors: | Wang Xiang-Bin Zhou Bing Yin Chang-Cheng Lin Qing Huang Hua-Liang |
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Institution: | Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing, 100101, China. |
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Abstract: | Antibody reshaping is an effective way to reduce the immunogenicity while maintaining or improving the affinity of murine antibodies. This paper describe a new in vitro approach for rapidly reshaping murine antibodies by combining DNA shuffling with ribosome display. With the new method, a reshaping anti-4-1BB single-chain antibody (scFv), Re-4B4-1 scFv, which bound to its antigen (4-1BB) specifically and strongly, was selected from a reshaping library. These results proved definitely the feasibility of the new designed approach for antibody reshaping. |
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