Assessment of the genetic relatedness of barley accessions (Hordeum vulgare s.l.) resistant to soil-borne mosaic-inducing viruses (BaMMV, BaYMV, BaYMV-2) using RAPDs |
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Authors: | F Ordon A Schiemann W Friedt |
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Institution: | (1) Institute of Crop Science and Plant Breeding I, Justus-Liebig-University, Ludwigstr. 23, D-35390 Giessen, Germany, DE |
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Abstract: | Thirty-six Hordeum vulgare varieties and 12 H. spontaneum germplasms originating from different parts of the world and showing different reactions to the barley yellow mosaic virus
complex (BaMMV, BaYMV, BaYMV-2) were analyzed for genetic similarity using RAPDs. On the basis of an analysis of 20 selected
RAPD-primers corresponding to 544 bands genetic similarity according to Nei and Li (1979) was estimated to be between 0.685
and 0.964. Associations between the 48 genotypes were calculated using UPGMA-clustering and principal coordinate analysis.
By applying these methods we were able to separate H. spontaneum accessions from H. vulgare varieties, and within these groups all the genotypes were clustered correctly according to their origin. Consequently, RAPD
analysis can be considered a very useful and efficient tool for the fast estimation of genetic relationships in barley. The
correlation between genetic similarity with respect to German varieties and adaptation of exotic barley varieties to German
growing conditions is discussed.
Received: 21 May 1996 / Accepted: 5 July 1996 |
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Keywords: | Hordeum vulgare s l Barley yellow mosaic disease Resistance RAPDs Genetic similarity |
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