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Studies on the localization and expression of nitric oxide synthase using histochemical techniques |
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| Authors: | P. J. Norris I. G. Charles C. A. Scorer P. C. Emson |
| Affiliation: | (1) MRC Molecular Neuroscience Group; Department of Neurobiology, The Babraham Institute, CB2 4AT Babmham, Cambridge, UK;(2) The Wellcome Foundation, Wellcome Research Laboratories, Langley Court, South Eden Park Road, BR3 3BS Beckenham, Kent, UK;(3) Present address: Department of Molecular and Cell Biology, Wyeth Research (UK) Ltd, Huntercombe Lane South, SL6 0PH Taplow, Maidenhead, Berks, UK |
| Abstract: | Summary This review provides an update on the variety of histochemical techniques available for the cellular localization and expression of nitric oxide synthase in formalin-fixed tissue sections. The techniques of immunohistochemistry and NADPH-diaphorase histochemistry are discussed and the suitability of various types of probes and reporters which are useful forin situ detection of nitric oxide synthase mRNA expression are assessed. Figures are also included which illustrate the techniques described and protocols forin situ hybridization and NADPH-diaphorase histochemistry. |
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