The home stretch,a first analysis of the nearly completed genome of Rhodobacter sphaeroides 2.4.1 |
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| Authors: | Mackenzie Chris Choudhary Madhusudan Larimer Frank W Predki Paul F Stilwagen Stephanie Armitage Judith P Barber Robert D Donohue Timothy J Hosler Jonathan P Newman Jack E Shapleigh James P Sockett R Elizabeth Zeilstra-Ryalls Jill Kaplan Samuel |
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| Institution: | (1) Department of Microbiology and Molecular Genetics, University of Texas-Houston Medical School, 6431 Fannin St., Houston, TX 77030, USA;(2) Life Sciences Division, 1060 Commerce Park, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA;(3) DOE Joint Genome Institute, 2800 Mitchell Drive, B400, Walnut Creek, CA 94598, USA;(4) Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK;(5) Department of Biological Sciences, University of Wisconsin-Parkside, Greenquist Hall, Kenosha, WI 53141, USA;(6) Bacteriology Department, University of Wisconsin-Madison, 1550 Linden Drive, Madison, WI 53706, USA;(7) Department of Biochemistry, University of Mississippi Medical Center, 2500 N. State St., Jackson, MS 39216, USA;(8) Department of Microbiology, Wing Hall, Cornell University, Ithaca, NY 14853-8101, USA;(9) Institute of Genetics, University of Nottingham, QMC, Nottingham, NG7 2UH, UK;(10) Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA;(11) Department of Microbiology and Molecular Genetics, University of Texas-Houston Medical School, 6431 Fannin St., Houston, TX 77030, USA |
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| Abstract: | Rhodobacter sphaeroides 2.4.1 is an α-3 purple nonsulfur eubacterium with an extensive metabolic repertoire. Under anaerobic conditions, it is able
to grow by photosynthesis, respiration and fermentation. Photosynthesis may be photoheterotrophic using organic compounds
as both a carbon and a reducing source, or photoautotrophic using carbon dioxide as the sole carbon source and hydrogen as
the source of reducing power. In addition, R. sphaeroides can grow both chemoheterotrophically and chemoautotrophically. The structural components of this metabolically diverse organism
and their modes of integrated regulation are encoded by a genome of ∼4.5 Mb in size. The genome comprises two chromosomes
CI and CII (2.9 and 0.9 Mb, respectively) and five other replicons. Sequencing of the genome has been carried out by two groups,
the Joint Genome Institute, which carried out shotgun-sequencing of the entire genome and The University of Texas-Houston
Medical School, which carried out a targeted sequencing strategy of CII. Here we describe our current understanding of the
genome when data from both of these groups are combined. Previous work had suggested that the two chromosomes are equal partners
sharing responsibilities for fundamental cellular processes. This view has been reinforced by our preliminary analysis of
the virtually completed genome sequence. We also have some evidence to suggest that two of the plasmids, pRS241a and pRS241b
encode chromosomal type functions and their role may be more than that of accessory elements, perhaps representing replicons
in a transition state.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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| Keywords: | complexity flagella gene duplication genome heme biosythesis nitrogen oxide reductase scaffold sequencing sigma factors terminal oxidases |
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