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Cloning of a Novel Rat Gene, DB83, That Encodes a Putative Membrane Protein |
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| Authors: | Nakadai, Tomoyoshi Kishimoto, Toshihiko Kokura, Kenji Ohkawa, Noriaki Makino, Yasutaka Muramatsu, Masami Tamura, Taka-aki |
| Affiliation: | 1Department of Biology, Faculty of Science, Chiba University Yayoi-cho, Inage-ku, Chiba 263-8522, Japan 2Biomedical Research and Development Department, Sumitomo Electric Industries, Limited Taya-cho, Sakae-ku, Yokohama, Japan 3Department of Biochemistry, Saitama Medical School Morohongo 38, Moroyama 350-0495, Saitama, Japan 4CREST Japan Science Technology Corporation |
| Abstract: | Using a partial cDNA sequence and a 5'-RACE technique, we isolateda novel cDNA from rat liver referred to as DB83. DB83 had fourhydrophobic trans-membrane domains and one N-myristoylationsite as well as multiple possible phosphorylation sites. Thedb83 gene was highly expressed in the liver and significantlyin brain, lungs and kidneys. We suggest that DB83 is a tissue-specificputative membrane protein. |
| Keywords: | cDNA cloning membrane protein tissue-specific gene expression |
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