| 土壤中PHA高产菌株的分离及鉴定 |
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| 引用本文： | 王丹,巩春甫,陈扬威,曾丽萍,何浪.土壤中PHA高产菌株的分离及鉴定[J].微生物学杂志,2010,30(6):93-96. |
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| 作者姓名： | 王丹 巩春甫 陈扬威 曾丽萍 何浪 |
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| 作者单位： | [1]成都医学院生物医学系,四川成都610083 [2]成都医学院基础医学院,四川成都610083 |
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| 基金项目： | 成都医学院校级科研课题项目 |
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| 摘 要： | 从成都某炼油厂油罐和排污口附近土壤中分离到1株PHA(聚羟基烷酸)的高产菌株,最高含量为19.25%。根据形态特征观察、生理生化特征鉴定及16S rDNA序列比对,结果表明该菌株为蜡状芽胞杆菌。并对其发酵条件进行初步探索,确定培养的最适温度为37℃,最适pH值为7.0,为下一步构建基因工程菌和优化发酵条件提供了理论基础。
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| 关 键 词： | PHA 菌株分离 鉴定 发酵条件 |
Isolation and Identification of PHA High-Producing Strain from Soil |
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| WANG Dan,GONG Chun-fu,CHEN Yang-wei,ZENG Li-ping,HE Lang.Isolation and Identification of PHA High-Producing Strain from Soil[J].Journal of Microbiology,2010,30(6):93-96. |
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| Authors: | WANG Dan GONG Chun-fu CHEN Yang-wei ZENG Li-ping HE Lang |
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| Institution: | 1.Dept.of Biomedicine,of,2.Sch.of Basic Med.of Chengdu Medical College,Chengdu 610083) |
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| Abstract: | A polyhydroxyalkanoate（PHA） high-yielding strain was isolated and purified from soil samples collected from neighboring site of oil storage tanks and the sewage outfall of a refinery in Chengdu,and the highest content of PHA was 19.25%（W/DW）.Based on the morphological observation,physiological and biochemical characteristics and the result of sequences comparison and verification of 16S rDNA,the strain was identified as Bacillus cereus.Preliminary experiment of its fermentation conditions was confirmed that the optimal culture temperature and pH were 37 ℃,and 7.0.These had provided a theoretical basis for further study on the construction of building a recombinant engineering strain that could produce PHA and the optimization of the fermentation conditions. |
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| Keywords: | polyhydroxyalkanoate（PHA） strain isolation identification fermentation conditions |
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