Roles of Myosin Va and Rab3D in Membrane Remodeling of Immature Secretory Granules |
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Authors: | Tanja Kögel Hans-Hermann Gerdes |
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Institution: | (1) Department of Biomedicine, University of Bergen, Jonas Lies Vei 91, 5009 Bergen, Norway; |
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Abstract: | Neuroendocrine secretory granules (SGs) are formed at the trans-Golgi network (TGN) as immature intermediates. In PC12 cells, these immature SGs (ISGs) are transported within seconds to
the cell cortex, where they move along actin filaments and complete maturation. This maturation process comprises acidification-dependent
processing of cargo proteins, condensation of the SG matrix, and removal of membrane and proteins not destined to mature SGs
(MSGs) into ISG-derived vesicles (IDVs). We investigated the roles of myosin Va and Rab3 isoforms in the maturation of ISGs
in neuroendocrine PC12 cells. The expression of dominant-negative mutants of myosin Va or Rab3D blocked the removal of the
endoprotease furin from ISGs. Furthermore, expression of mutant Rab3D, but not of mutant myosin Va, impaired cargo processing
of SGs. In conclusion, our data suggest an implication of myosin Va and Rab3D in the maturation of SGs where they participate
in overlapping but not identical tasks. |
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