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Androgen receptor complex binding in murine skeletal muscle nuclei |
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| Authors: | G Vollmer A Haase K Eisele |
| Institution: | Physiologisch-chemisches Institut der Universität 7400 Tübingen, FRG. |
| Abstract: | Examination of binding of androgen-receptor complexes from murine skeletal muscle cytosol was performed by modified nuclear retention assay and modified nuclear acceptor assay. The experiments showed the binding of androgen-receptor complexes to the nuclear acceptor sites to be a cooperative process. Hill analysis of the data obtained resulted in a Hill coefficient of 3,6. The apparent dissociation constant for binding of cytosolic 3H]-testosterone-receptor complexes to nuclei was found to be in the range of KD = 6 ? 8 × 10?11 M. The nuclear matrix was able to bind androgen-receptor complexes in a saturable way, too. |
| Keywords: | PMSF phenylmethylsulfonylfluoride TEMS 10 mM Tris-HCl pH 7 4 containing 2 mM EDTA 2 mM 2-mercaptoethanol 0 25 M sucrose and 0 1 mM PMSF TMKS NRA nuclear retention assay NAA nuclear acceptor assay DCC dextran coated charcoal (4 % charcoal Norit A 0 1 % dextran T60 and 0 1 % gelatine in TEMS) |
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