| 松科植物基因组总DNA提取方法的比较 |
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| 引用本文： | 魏志刚,王艳敏,杨传平.松科植物基因组总DNA提取方法的比较[J].生物技术通讯,2007,18(2):263-266. |
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| 作者姓名： | 魏志刚 王艳敏 杨传平 |
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| 作者单位： | 东北林业大学,林木遗传育种与生物技术教育部重点实验室,黑龙江,哈尔滨,150040 |
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| 基金项目： | 国家高技术研究发展计划(863计划);东北林业大学校科研和教改项目 |
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| 摘 要： | 目的:研究对比了用不同方法提取红松、樟子松和红皮云杉等3种松科植物叶片基因组DNA的效果,以寻找适合提取松科植物叶片基因组DNA的方法。方法:分别比较了用传统的CTAB法、SDS法以及3种改良的CTAB法提取的上述3种松科植物叶片基因组DNA的电泳、纯度和酶切效果。结果:采用不同方法提取的3种松科植物叶片基因组DNA的质量差异较大。结论:常规CTAB法和SDS法无法提取出高质量松科植物基因组DNA,而改良后的CTAB法的提取效果较好。
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| 关 键 词： | 基因组DNA 松科植物 DNA提取 |
| 文章编号： | 1009-0002（2007）02-0263-04 |
| 修稿时间： | 2006-08-04 |
Comparison of Five Methods of DNA Extraction from Pinaceae Plants |
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| WEI Zhi-gang,WANG Yan-min,YANG Chuan-ping.Comparison of Five Methods of DNA Extraction from Pinaceae Plants[J].Letters in Biotechnology,2007,18(2):263-266. |
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| Authors: | WEI Zhi-gang WANG Yan-min YANG Chuan-ping |
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| Institution: | Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University Harbin 150040, China |
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| Abstract: | Objective:In order to find the suit method of genome DNA extraction from Pinaceae,the different methods of genome DNA extraction from there kind of Pinaceae plants(Pinus koraiensis,Pinus sylvestris var.mongolica,Picea koraiensis)were studied.Methods:The result of electrophoresis,purity and enzyme digesting of genome DNA of three kind of Pinaceae were compared.Results:The quality of DNA by different methods of genome DNA extraction was different.Conclusion:The results showed that the improving CTAB was better than conventional CTAB and SDS. |
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| Keywords: | genomic DNA Pinaceae plants DNA extraction |
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