Indomethacin inhibits cholera toxin-induced cyclic AMP accumulation in Chinese hamster ovary cells |
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Authors: | Johnny W Peterson William D Berg Laura G Ochoa |
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Institution: | Department of Microbiology, University of Texas Medical Branch, Galveston, TX 77550, U.S.A. |
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Abstract: | Abstract Indomethacin was examined for its capacity to inhibit increases in adenosine-3',5'-monophosphate (cAMP) concentrations in Chinese hamster ovary (CHO) cells treated with cholera toxin. When added to the culture medium 1 h prior to cholera toxin (100 ng/ml), indomethacin (500 μg/ml) exhibited maximum protection against the typical increase in cAMP. Application of indomethacin at the same time as cholera toxin or up to 3 h after the toxin progressively decreased the drug's capacity to block further increases in cAMP. The drug appeared to block adenylate cyclase activity because addition of forskolin to drug-treated cells did not elicit a cAMP response. Binding of 125I-labeled cholera toxin to indomethacin-treated cells was also reduced by at least 50%. These data indicate that indomethacin's inhibitory effect on cAMP formation in cholera toxin-treated cells could be explained by its capacity to alter adenylate cyclase activity and cholera toxin binding. |
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Keywords: | Indomethacin Cyclic AMP Chinese hamster ovary Cholera toxin |
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