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| 体细胞克隆牛和转基因体细胞克隆牛的遗传学分析(英) | |
| 引用本文： | 黄银花,徐公瑾,杜志强,胡晓湘,张磊,涂剑锋,程雪波,李宁. 体细胞克隆牛和转基因体细胞克隆牛的遗传学分析(英)[J]. 生物化学与生物物理进展, 2004, 31(8): 699-704 |
| 作者姓名： | 黄银花 徐公瑾 杜志强 胡晓湘 张磊 涂剑锋 程雪波 李宁 |
| 作者单位： | 1. 中国农业大学农业与生物技术国家重点实验室,北京,100094 2. 中国农业大学农业与生物技术国家重点实验室,北京,100094;延边农学院动物科学院,龙井,133400 3. 中国农业大学农业与生物技术国家重点实验室,北京,100094;江西农业大学动物科技学院,南昌,330045 |
| 基金项目： | 国家高技术“863”计划资助项目(2002AA206111)和国家重点基础研究发展规划项目(G20000161). |
| 摘 要： | 分析了来自同一细胞系的体细胞克隆牛甜甜、庆庆、浒娃及来源同一培养转基因体细胞系转基因体细胞克隆牛九妹、乐娃和1个妊娠8个月转基因流产胎牛8C2以及随机抽取的1头鲁西黄牛(LX)、1头褐斯坦牛(HS)在24个微卫星位点标记牛的基因型.结果表明24个多态位点均表现出多态，等位基因数为1～5个，平均为3.17个.根据网上公布的数据，按其最高频率计算，甜甜、庆庆、浒娃、九妹、乐娃、8C2与培养细胞系、转基因细胞系间匹配概率为1.17×10－36，根据本研究观察到的数据计算，匹配概率为1.90×10－23；而与随机抽取的1头鲁西黄牛及褐斯坦牛的基因型分别在23和20个位点上完全不同. |
| 关 键 词： | 克隆牛，遗传学，分析 |
| 收稿时间： | 2004-03-08 |
| 修稿时间： | 2004-04-28 |
Genetic Analysis of Cattle Cloned From Somatic Cells and Gene Transferred Somatic Cells |
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| HUANG Yin-Hu,XU Gong-Jin,DU Zhi-Qiang,HU Xiao-Xiang,ZHANG Lei,TU Jian-Feng,CHENG Xue-Bo and LI Ning. Genetic Analysis of Cattle Cloned From Somatic Cells and Gene Transferred Somatic Cells[J]. Progress In Biochemistry and Biophysics, 2004, 31(8): 699-704 | |
| Authors: | HUANG Yin-Hu XU Gong-Jin DU Zhi-Qiang HU Xiao-Xiang ZHANG Lei TU Jian-Feng CHENG Xue-Bo LI Ning |
| Affiliation: | State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China;State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China;Department of Animal Science, College of Agriculture, Yanbian University, Longjing 133400, China;State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China;State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China;State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China;State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China;College of Animal Science, Jiangxi Agricultural University, Nanchang 330045, China;State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China;State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China |
| Abstract: | Three cloned cattle from the same somatic cell line (TT, QQ, XW) and three transferred cloned cattle from the same gene transferred cell line (JM, LW, 8C2), together with two randomly selected cattle, one LUXI (LX) and one Holstein (HS), were genetically analyzed using 24 fluorescent microsatellite markers. There were 1 ～ 5 alleles at 24 loci,the average being 3. 17. Among TT, QQ, XW, JM, LW, 8C2, the somatic cell line and the gene transferred somatic cell line, the probabilities of matching (PM) between individuals were 1.17 × 10-36 using allele frequencies obtained from public data on internet and 1.90 × 10 -23 by defining the allele frequencies at each locus as the reciprocal of the number of alleles at that locus. In addition, their genotypes differed from those of the randomly selected LX and HS at 23 and 20marker loci respectively. |
| Keywords: | cloned cattle genetic analysis gene transferred somatic cell line |
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