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Cloning of caf1 +, caf2 + and caf4 + from Schizosaccharomyces pombe : their involvement in multidrug resistance, UV and pH sensitivity |
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| Authors: | Z Benko M Sipiczki A M Carr |
| Institution: | Trafford Centre, University of Sussex, Falmer Brighton, BN1 9RY, UK e-mail: peterdora@tigris.klte.hu Fax: +36-52-348550, GB Department of Genetics, University of Debrecen PO Box 56, H-4010 Debrecen, Hungary, HU MRC Cell Mutation Unit, University of Sussex Falmer, Brighton, BN1 9RR, UK, GB |
| Abstract: | We previously identified four nuclear genes (caf1 + to caf4 +) in Schizosaccharomyces pombe, mutations in which can confer caffeine resistance. Here we report the cloning and sequencing of caf1 +, caf2 + and caf4 +. All three genes are allelic to genes (hba1 + , crm1 + and trr1 + , respectively) involved in multidrug resistance mechanisms or in stress response systems. In agreement with this the caffeine-resistant mutants caf1(hba1)-21, caf2(crm1)-3 and caf4(trr1)-83 are also resistant to brefeldin. Disruption of caf1(hba1) + and caf4(trr1) + makes cells sensitive to high pH. The overlapping ranges of pleiotropic effects and the genetic interaction detected between caf1(hba1) + and caf2(crm1) + suggest that the three genes function in interlinked systems. |
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