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Conjugation transfer of the plasmid pAMbeta1 into various Bacillus species
Authors:N F Riabchenko  L M Rumer  V A Livshits  Iu I Kozlov
Abstract:Streptococcal broad host range plasmid pAM beta 1 was transferred by a conjugation-like process from Streptococcus faecalis to 13 strains of different Bacilli species. In intraspecies matings the frequencies of transfer of pAM beta 1 varied from 2.10(-5) to 1.10(-8). As it was shown by comparative analysis the frequency of transfer and stability of the maintainance of plasmid pAM beta 1 in Bacilli were not connected. Molecular weight and restriction pattern of pAM beta 1 DNA isolated from Bacilli were the same as those of pAM beta 1 DNA from Streptococcal donor strain.
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