Stimulation by Bradykinin, Angiotensin II, and Carbachol of the Accumulation of Inositol Phosphates in PC-12 Pheochromocytoma Cells: Differential Effects of Lithium Ions on Inositol Mono-and Polyphosphates |
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Authors: | Dietrich van Calker Karin Assmann Waldemar Greil |
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Affiliation: | Psychiatric Hospital, University of Munich, F.R.G. |
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Abstract: | Rat PC-12 pheochromocytoma cells respond to stimulation with bradykinin, angiotensin II, and carbachol with an increased formation of labeled inositol phosphates after preincubation of the cells with [3H]inositol. Li+ potentiates greatly the agonist-induced increase in amount of inositol mono-, bis-, and trisphosphate but not the increase in amount of inositol tetrakisphosphate. Separation of the isomers of inositol trisphosphate shows that the lithium-induced increase in amount of inositol trisphosphate is due to potentiation evoked by lithium of the accumulation of inositol-1,3,4-trisphosphate. |
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Keywords: | Li+ Muscarinic acetylcholine receptor Bradykinin Angiotensin II Phosphoinositides PC-12 pheochromocytoma cells |
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