转抗虫基因欧美黑杨离体快繁技术研究

以抗虫欧美黑杨的叶，带腋芽茎段为外植体进行离体快繁技术研究。最佳接种时间为8月份，新芽生长迅速。基本培养基为MS，较适初培养基为MS＋6－BA0.5mg/L（以下单位同）＋NAA0.01mg/，附加30g/L，蔗糖，7g/L琼脂。愈伤组织诱导并同时分化出新芽培养基为MS＋6－BA1.5 NAA0.3，附加40g/L蔗糖，6g/L琼脂。继代增殖培养基为MS 6-BA1.0 NAA0.1 GA2.0，附加30g/L蔗糖，5g/L琼脂。生根培养基为MS＋IBA2．0。

In vitro micropropagation of insect-resistant transgenic Populus euramericana

Shoots and axillary buds were induced in vitro from apexes,stem segments and leaves of insect-resistant transgenic Populus euramericana .August is the best inoculated time and new buds grew rapidly.Basic medium is MS medium.The proper initial culture medium is MS+6 BA 0.5 mg/L+NAA 0.01 mg/L,buds were regenerated from the explants.Calluses were induced and meanwhile cluster adventitious buds were formed on the MS basic containing 1.5 mg/L 6 BA and 0.3 mg/L NAA,high sugar concentration (40 mg/L) is suitable for calluses inducing and new buds forming.The medium for multiplication is MS+6 BA 1.0 mg/L+NAA 0.1 mg/L+GA 2.0 mg/L.The rooting medium is MS+IBA 2.0 mg/L.