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Structural and biological characterization of Nattectin, a new C-type lectin from the venomous fish Thalassophryne nattereri |
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| Authors: | Lopes-Ferreira Mônica Magalhães Geraldo Santana Fernandez Jorge Hernandez Junqueira-de-Azevedo Inácio de Loiola M Le Ho Paulo Lima Carla Valente Richard H Moura-da-Silva Ana Maria |
| Affiliation: | a Laboratório Especial de Toxinologia Aplicada (CEPID/FAPESP), Instituto Butantan, Avenida Vital Brazil, 1500. Butantan. São Paulo, São Paulo. Brazil b Laboratório de Imunopatologia, Instituto Butantan, São Paulo, São Paulo, Brazil c Laboratório Nacional de Computação Científica, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Brazil d Centro de Biotecnologia, Instituto Butantan, São Paulo, São Paulo, Brazil e Laboratório de Toxinologia, Instituto Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil |
| Abstract: | Lectins are glycan-binding receptors that recognize glycan epitopes on foreign pathogens and in the host systems. They can be involved in functions that include innate immunity, development, immune regulation and homeostasis. Several lectins have been purified and characterized from fish species. In this work, using cation-exchange chromatography, a galactose-specific lectin belonging to the family of C-type lectins was isolated from the venom of the Brazilian venomous fish Thalassophryne nattereri. Nattectin is a basic, non-glycosilated, 15 kDa monomeric protein. It exhibits hemagglutination activity that is independent of Ca2+. We also demonstrated a lectin activity for Nattectin in the innate immune system, especially in neutrophil mobilization in mice, indicating that marine organisms are source of immunomodulator agents. |
| Keywords: | Nattectin C-type lectin Galactose Inflammation Fish venom Thalassophryne nattereri |
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