Sequencing and analysis of the cos region of the lactococcal bacteriophage c2 |
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Authors: | Mark W Lubbers Lawrence J H Ward Tom P J Beresford Brion D W Jarvis and Audrey W Jarvis |
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Institution: | (1) New Zealand Dairy Research Institute, Private Bag, 11 029 Palmerston North, New Zealand;(2) Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand;(3) Present address: National Dairy Products Research Centre, Moorepark, Fermoy Co., Cork, Ireland |
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Abstract: | The cohesive termini of the DNA genome of the lactococcal bacteriophage c2 were directly sequenced and appeared to be complementary, non-symmetrical, 9-nucleotide single-stranded, 3 extended DNAs, with the following sequence: 5-GTTAGGCTT-3 3-CAATCCGAA-5. DNA located on either side of the cohesive ends was sequenced and several repeats and a region with the potential for a DNA bend were found. Previously sequenced cos regions of 13 other bacteriophages were also examined for similar sequence features. All of the bacteriophages from gram-positive hosts had 3 extended DNA termini, in contrast to the bacteriophages from gram-negative hosts, which had 5 extended DNA termini. All bacteriophages had a region of dyad symmetry close to the cohesive termini. A 7.3 kb DNA fragment of the c2 genome containing the cos sequences was cloned; transduction experiments demonstrated that these cloned sequences could act as a substrate for packaging enzymes of phage c2. |
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Keywords: | Cohesive ends Lytic phage Prolate-headed phage DNA packaging 3 Extension" target="_blank">gif" alt="prime" align="BASELINE" BORDER="0"> Extension |
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