Isolation and characterization of squalene synthase cDNA fromcentella asiatica (L) urban |
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| Authors: | Ok -Tae Kim Nak -Sul Seong Min -Young Kim Baik Hwang |
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| Affiliation: | (1) National Institute of Crop Science, RDA, 441-857 Suwon, Korea;(2) Department of Biology and Institute of Plant Resources, Chonnam National University, 500-757 Cwangju, Korea |
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| Abstract: | We have cloned and characterized a gene for squalene synthase (SQS) fromCentella asiatica (L) Urban, a species that produces a large quantity of triterpene saponins such as asiaticoside and madecassoside. Its full-length
cDNA clone was isolated by RACE PCR. The sequence ofpSQS contains an open reading frame of 1248 nucleotides, which code for 416 amino acids with a molecular mass of 47.3 kDa. Southern
analysis revealed that one copy might exist in the C.asiatica genome. We also determined that 0.1 mM methyl jasmonate was sufficient to up-regulate those levels ofCaSQS mRNA. |
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| Keywords: | asiaticoside Centella asiatica squalene synthase triterpene saponins |
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