Exploiting co-linearity among grass species to map the <Emphasis Type="Italic">Aegilops ventricosa-</Emphasis>derived <Emphasis Type="Italic">Pch1</Emphasis> eyespot resistance in wheat and establish its relationship to <Emphasis Type="Italic">Pch2</Emphasis> |
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Authors: | C Burt P Nicholson |
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Institution: | (1) John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK; |
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Abstract: | Introgressions into wheat from related species have been widely used as a source of agronomically beneficial traits. One such
example is the introduction of the potent eyespot resistance gene Pch1 from the wild relative Aegilops ventricosa onto chromosome 7DL of wheat. In common with genes carried on many other such introgressions, the use of Pch1 in commercial wheat varieties has been hindered by linkage drag with yield-limiting traits. Attempts to break this linkage
have been frustrated by a lack of co-dominant PCR markers suitable for identifying heterozygotes in F2 populations. We developed conserved orthologous sequence (COS) markers, utilising the Brachypodium distachyon (Brachypodium) genome sequence, to provide co-dominant markers in the Pch1 region. These were supplemented with previously developed sequence-tagged site (STS) markers and simple sequence repeat (SSR)
markers. Markers were applied to a panel of varieties and to a BC6 F2 population, segregating between wheat and Ae. ventricosa over the distal portion of 7DL, to identify recombinants in the region of Pch1. By exploiting co-linearity between wheat chromosome 7D, Brachypodium chromosome 1, rice chromosome 6 and sorghum chromosome
10, Pch1 was located to an interval between the flanking markers Xwg7S and Xcos7-9. Furthermore candidate gene regions were identified in Brachypodium (364 Kb), rice (178 Kb) and sorghum (315 Kb) as a prelude
to the map-based cloning of the gene. In addition, using homoeologue transferable markers, we obtained evidence that the eyespot
resistances Pch1 and Pch2 on chromosomes 7D and 7A, respectively, are potentially homoeoloci. It is anticipated that the COS marker methodology could
be used for the identification of recombinants in other introgressions into wheat from wild relatives. This would assist the
mapping of genes of interest and the breaking of deleterious linkages to enable greater use of these introgressions in commercial
varieties. |
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