| 白念珠菌CaGDT1基因的功能研究 |
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| 引用本文： | 王军军,王雅楠,徐大勇,蒋伶活.白念珠菌CaGDT1基因的功能研究[J].微生物学杂志,2016(2):14-19. |
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| 作者姓名： | 王军军 王雅楠 徐大勇 蒋伶活 |
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| 作者单位： | 江南大学 生物工程学院 粮食发酵工艺与技术国家工程实验室，江苏 无锡 214122,江南大学 生物工程学院 粮食发酵工艺与技术国家工程实验室，江苏 无锡 214122,江南大学 生物工程学院 粮食发酵工艺与技术国家工程实验室，江苏 无锡 214122,江南大学 生物工程学院 粮食发酵工艺与技术国家工程实验室，江苏 无锡 214122 |
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| 基金项目： | 国家自然科学基金项目 (81371784)；江南大学自主研究计划重点项目(JUSRP51313B) |
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| 摘 要： | 人类跨膜蛋白TMEM165与酿酒酵母Sc Gdt1均属于阳离子/钙离子交换器家族的成员,在本研究中,通过序列比对在白念珠菌中发现了Sc GDT1的同源基因Ca GDT1,表型互补实验显示Ca GDT1基因的表达能够抑制Sc GDT1基因缺失所造成的钙离子敏感性,证明Ca GDT1是Sc GDT1的同功基因。此外,通过同源重组原理敲除了Ca GDT1的2个等位基因。表型筛选结果表明gdt1/gdt1缺失株对钙离子、细胞壁和内质网3种胁迫均不敏感,而对酮康唑和特比萘芬2种抗真菌药物具有耐受性。
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| 关 键 词： | 白念珠菌 CaGDT1 基因敲除 耐药性 |
Function of CaGDT1 Gene in Candida albicans |
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| WANG Jun-jun,WANG Ya-nan,XU Da-yong and JIANG Ling-huo.Function of CaGDT1 Gene in Candida albicans[J].Journal of Microbiology,2016(2):14-19. |
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| Authors: | WANG Jun-jun WANG Ya-nan XU Da-yong and JIANG Ling-huo |
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| Institution: | Coll. of Bio Engin., Jiangnan Uni., State Lab. of Grain Ferment. Technol. & Tech., Wuxi 2141222,Coll. of Bio Engin., Jiangnan Uni., State Lab. of Grain Ferment. Technol. & Tech., Wuxi 2141222,Coll. of Bio Engin., Jiangnan Uni., State Lab. of Grain Ferment. Technol. & Tech., Wuxi 2141222 and Coll. of Bio Engin., Jiangnan Uni., State Lab. of Grain Ferment. Technol. & Tech., Wuxi 2141222 |
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| Abstract: | Human transmembrane protein 165 (TMEM165) and Saccharomyces cerevisiae ScGdt1 belong to the members of cation/Ca2+ exchanger family. In this study, homologous gene of CaGDT1 of ScGDT1 was found in Candida albicans through sequence comparison. Phenotypic mutual complementary experiment showed that the expression of CaGDT1 could suppress the calcium ion sensitivity caused by ScGDT1 deletion, proved that CaGDT1 and ScGDT1 are analogous genes. In addition, two alleles of CaGDT1 were knockout through homologous recombination principle. The results of phenotypic screening suggested that the gdt1/gdt1 deletion strain was all not sensitive to triple stresses of calcium ion, cell wall, ER, however, was tolerant against antifungal drugs, ketoconazole and terbinafine. |
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| Keywords: | Candida albicans CaGDT1 gene knockout drug resistance |
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