Variation and Estimation of the Differential Absorption Coefficient of P-700 in Spinach Photosystem I Preparations |
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Authors: | Sonoike Kintake; Katoh Sakae |
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Institution: | 1Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN) Wako, Saitama, 351-01 Japan
2Department of Biology, Faculty of Sciences, University of Tokyo Hongo, Bunkyo-ku, Tokyo, 113 Japan |
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Abstract: | Treatment of spinach Photosystem I particles with detergentsinduced an apparent blue shift of chlorophyll at 690 nm inthe difference spectrum of P-700. As a consequence of the bandshift, the differential absorption coefficient of P-700 wasincreased from 63 to 87 mM1 .cm1. A curve waspresented, with which changes in the apparent differential absorptioncoefficient of P-700 can be estimated by measuring magnitudesof light-induced absorption decreases at 680 nm and 700 nm.The curve was compared with that for cyanobacterial preparationsand the mechanism of the detergent-induced band shift was discussed. (Received July 11, 1990; Accepted August 23, 1990) |
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