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Mutants of Rhodopseudomonas sphaeroides useful in genetic analysis |
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| Authors: | W. R. Sistrom A. Macaluso R. Pledger |
| Affiliation: | (1) Department of Biology, University of Oregon, 97403 Eugene, OR, USA;(2) Present address: Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, MA, USA |
| Abstract: | Two kinds of mutants of Rhodopseudomonas sphaeroides that should be useful in extending genetic analysis of this organism have been isolated. One is deficient in recombination and has been used to isolate derivatives of the plasmid R 68.45 which incorporate chromosomal genes of R. sphaeroides. The other is apparently defective in a DNA restriction enzyme; transfer of plasmid borne chromosomal genes of R. sphaeroides from Escherichia coli back to R. sphaeroides is greatly enhanced in these mutants.In memory of R. Y. Stanier |
| Keywords: | Rhodopseudomonas sphaeroides R-prime plasmids Recombination Restriction deficient |
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