结直肠癌组织miR-137-3p、miR-410-3p表达水平与上皮间充质转化和预后的关系分析

摘要 目的：探讨结直肠癌（CRC）组织微小RNA-137-3p（miR-137-3p）、微小RNA-410-3p（miR-410-3p）的表达与上皮间充质转化（EMT）和预后的关系。方法：选取2017年2月至2019年2月本院收治的115例接受CRC根治术治疗的患者，采用实时荧光定量聚合酶链式反应（qRT-PCR）检测癌组织及癌旁组织中miR-137-3p、miR-410-3p表达、EMT标志物E-钙粘蛋白（E-cadherin）、波形蛋白（Vimentin）的mRNA表达并进行相关性分析，分析癌组织中miR-137-3p、miR-410-3p表达与CRC临床病理特征的关系。术后随访3年，采用Kaplan-Meier法分析miR-137-3p、miR-410-3p高表达和低表达患者的预后情况。结果：癌组织中miR-137-3p表达及E-cadherin mRNA表达水平低于癌旁组织，miR-410-3p表达及Vimentin mRNA表达水平高于癌旁组织（P＜0.05）。癌组织中miR-137-3p表达与E-cadherin mRNA表达水平、miR-410-3p表达与Vimentin mRNA表达水平分别呈正相关（P＜0.05），癌组织中miR-137-3p表达与Vimentin mRNA表达水平、miR-410-3p表达与E-cadherin mRNA表达水平分别呈负相关（P＜0.05）。miR-137-3p、miR-410-3p表达与TNM分期、肿瘤分化程度、淋巴结转移有关（P＜0.05）。Kaplan-Meier生存曲线分析显示，miR-137-3p低表达、miR-410-3p高表达患者3年累积生存率下降（P＜0.05）。结论：CRC组织中miR-137-3p表达下调、miR-410-3p表达上调，miR-137-3p、miR-410-3p表达水平与EMT密切相关，且miR-137-3p高表达、miR-410-3p低表达患者的预后更好。

Analysis of the Relationship between the Expression Levels of miR-137-3p and miR-410-3p in Colorectal Cancer Tissue and Epithelial Mesenchymal Transition and Prognosis

ABSTRACT Objective: To investigate the relationship between the expression of microRNA-137-3p (miR-137-3p) and microRNA-410-3p (miR-410-3p) in colorectal cancer (CRC) tissue and epithelial mesenchymal transition (EMT) and prognosis. Methods: 115 patients who received CRC radical surgery in our hospital from February 2017 to February 2019 were selected. Real time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-137-3p, miR-410-3p, and the mRNA expression of EMT markers E-cadherin, Vimentin in cancer tissues and adjacent tissues. The relationship between miR-410-3p expression and clinical pathological characteristics of CRC were analyzed. Follow up for 3 years after surgery, Kaplan Meier method was used to analyze the prognosis of patients with high and low expression of miR-137-3p and miR-410-3p. Results: The expression levels of miR-137-3p and E-Cadherin mRNA in cancer tissues were lower than those in adjacent tissues, while the expression levels of miR-410-3p and Vimentin mRNA were higher than those in adjacent tissues (P<0.05). The expression of miR-137-3p in cancer tissue was positively correlated with the expression level of E-cadherin mRNA, and the expression level of miR-410-3p was positively correlated with the expression level of Vimentin mRNA, respectively (P<0.05). The expression level of miR-137-3p in cancer tissue was negatively correlated with the expression level of Vimentin mRNA, and the expression level of miR-410-3p was negatively correlated with the expression level of E-cadherin mRNA, respectively (P<0.05). The expression of miR-137-3p and miR-410-3p was related to TNM staging, tumor differentiation, and lymph node metastasis (P<0.05). Kaplan Meier survival curve analysis showed that patients with low expression of miR-137-3p and high expression of miR-410-3p had a decreased 3-year cumulative survival rate (P<0.05). Conclusion: The expression of miR-137-3p is down regulated and miR-410-3p is up regulated in CRC tissues. The expression levels of miR-137-3p and miR-410-3p are closely related to EMT, and patients with high expression of miR-137-3p and low expression of miR-410-3p have better prognosis.