山西省不同地区柴胡根部可培养内生真菌的多样性分析

为研究柴胡（Bupleurum chinense DC.）根部可培养内生真菌的多样性，分析山西省不同地区柴胡根部可培养内生真菌多样性的差异性，建立柴胡根部内生真菌库。本文以山西省不同产地的柴胡根为材料，采用传统植物组织平板分离法对柴胡根部可培养内生真菌进行分离培养和分子鉴定。从25个不同产地的柴胡样品根部中共分离培养得到705株真菌，经分子生物学鉴定，其中695株真菌归属为4门14纲23目32科55属119种。晋南地区的优势菌属为镰刀菌属（Fusarium, 32.18%）、亚隔孢壳属（Didymella, 15.96%）、曲霉属（Aspergillus, 8.24%）、异茎点霉属（Paraphoma, 6.91%）、茎点球属（Phoma, 5.32%）、链格孢属（Alternaria, 5.05%），晋北地区的优势菌属为镰刀菌属（31.66%）、曲霉属（15.36%）、链格孢属（9.09%）、亚隔孢壳属（8.15%）、异茎点霉属（7.52%），亚隔孢壳属、曲霉属、链格孢属等优势属的相对丰度差异较大；晋南地区的优势物种为Didymella bellidis（11.44%）、三线镰刀菌（Fusarium tricinctum, 7.98%）、腐皮镰刀菌（Fusarium solani, 7.45%）、锐顶镰孢菌（Fusarium acuminatum, 5.85%）、烟曲霉（Aspergillus fumigatus, 5.85%），晋北地区的优势物种为锐顶镰孢菌（14.11%）、烟曲霉（11.91%）、三线镰刀菌（9.40%）、菊异茎点霉（Paraphoma chrysanthemicola, 5.64%）、D. bellidis（5.64%）、链格孢菌（Alternaria alternata, 5.64%），D. bellidis、锐顶镰孢菌、烟曲霉等优势种的相对丰度差异较大。差异性分析表明晋南地区的生物多样性显著高于晋北地区，D. bellidis、锐顶镰孢菌、烟曲霉等优势种的相对丰度差异较大，为进一步研究柴胡根内生真菌相对丰度差异较大的物种及其不同菌株对柴胡皂苷积累的影响提供参考。

Diversity of Culturable Endophytic Fungi from the Roots of Chinese Thorowax (Bupleurum chinense) in Different Regions of Shanxi Province

In order to investigate the diversity of culturable endophytic fungi in the roots of Chinese thorowax or radix bupleuri (Bupleurum chinense) DC. (Bupleurum chinense), the differences in culturable endophytic fungal diversity on the roots of B. chinense from different regions of Shanxi Province were analyzed, and established their endophytic fungal databank of B. chinense roots, and performed isolation, cultivation and molecular characterization of culturable endophytes in the roots of B. chinense, and the experimental material was collected from different production regions in Shanxi Province. 705 fungi isolated from the roots of B. chinense samples from 25 different locations were molecularly characterized, among them 695 fungal strains were assigned to 119 species belonging to 4 phyla, 14 classes, 23 orders, 32 families, and 55 genera. The dominant genera in the southern region of Shanxi Province were Fusarium(32.18%), Didymella(15.96%), Aspergillus(8.24%), Paraphoma(6.91%), Phoma(5.32%),Alternaria (5.05%), and the dominant genera in the northern region of Shanxi Province were Fusarium(31.66%), Aspergillus (15.36%), Alternaria (9.09%), Didymella(8.15%), and Paraphoma(7.52%). The relative abundance of dominant genera such as Didymella, Aspergillus and Alternaria varied greatly. Didymella bellidis(11.44%), Fusarium tricinctum(7.98%),Fusarium solani(7.45%), Fusarium. acuminatum (5.85%) and Aspergillus fumigatus (5.85%) were dominant species in southern Shanxi. The dominant species in northern Shanxi were Fusarium acuminatum (14.11%), Aspergillus fumigatus (11.91%), Fusarium tricinctum(9.40%), Paraphoma chrysanthemicola (5.64%), Didymella bellidis(5.64%), and Alternaria alternata(5.64%). The relative abundance of dominant species such as D. bellidis, F. acuminatum, and A. fumigatus varied greatly. The diversity analysis showed that the biodiversity of southern Shanxi was significantly higher than that of northern Shanxi, and the relative abundance of dominant species such as D. bellidis, F. acuminatum and A. fumigatus was significantly different. This study laid the foundation for further studies on the effects of species with large differences in the relative abundance of endophytic fungi in the root of B. chinense and different strains on the accumulation of saikosaponins.