利用超数排卵方法高效获取小鼠休眠胚胎

目的为提高小鼠休眠胚胎的制备效率,尝试用超数排卵方法获取小鼠休眠胚胎。方法超排小鼠在注射孕马血清促性腺激素(PMSG)后,按照是否注射抗孕马血清(anti-PMSG serum或A-PMSG)以及注射的不同时间分为5组:在母鼠合笼前注射A-PMSG、在母鼠见栓后注射A-PMSG、在见栓第四天卵巢摘除手术后注射A-PMSG、不注射A-PMSG的超排组和不超排组。结果见栓后注射抗PMSG组的平均出胚数(9.4枚/只)最高,显著高于其他实验组。结论常规超排处理结合注射抗PMSG血清法能有效提高小鼠休眠胚胎的回收率。

A New Method to Obtain Murine Dormant Embryos by Superovulation Combined with Anti-PMSG Serum

Objective In order to improve the preparation efficiency of mouse dormant embryos,the normal superovulation combined with anti-PMSG serum(A-PMSG) was applied to collect the dormant ones.Methods According to the different doses of A-PMSG injected into the mouse after injection of PMSG,the mice were divided into five groups: 1) injecting A-PMSG before mating,2) injecting A-PMSG after finding a vaginal plug,3) injecting A-PMSG after ovariectomy in the morning of day 4(day1= vaginal plug),4) superovulating without A-PMSG,and 5) the control one.Results The average number of recovered embryos(9.4) of the group injected with the A-PMSG after finding a vaginal plug,is significantly higher than the other groups(P<0.01).Conclusion Superovulation combined with anti-PMSG serum can improve the collecting efficiency of murine dormant embryos.