PCR analysis of the wheat varieties and near-isogenic wheat lines with the use of allele-specific primers for the <Emphasis Type="Italic">Gli-1</Emphasis> and <Emphasis Type="Italic">Glu-3</Emphasis> loci |
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| Authors: | A M Polishchuk S V Chebotar E M Blagodarova N A Kozub I A Sozinov Yu M Sivolap |
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| Institution: | 1.South Plant Biotechnology Center,Ukrainian Academy of Agrarian Sciences,Odessa,Ukraine;2.The Plant Breeding and Genetics Institute — National Center of Seed and Cultivar Investigation,Ukrainian Academy of Agrarian Sciences,Odessa,Ukraine;3.Institute of Plant Protection,Ukrainian Academy of Agrarian Sciences,Kyiv,Ukraine;4.DU “Institute of Food Biotechnology and Genomics,Ukrainian National Academy of Sciences,Kyiv,Ukraine |
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| Abstract: | The allelic characteristics of Gli-A1, Gli-B1, Gli-D1 and Glu-A3 loci of 14 bread wheat varieties and 6 near-isogenic wheat lines derived from the Bezosta 1 variety were found by the use
of PCR. The conformity between molecular-genetic and storage protein electrophoretic data was revealed: the GliA1.2 allele corresponds to the Gli-A1o and Gli-A1m allelic variants of gliadin blocks; the GliA1.1 PCR allele corresponds to the Gli-A1f, Gli-A1b and Gli-A1c variants of gliadin blocks; the GliB1.1 allele corresponds to the Gli-B1b and Gli-B1d allelic variants; and the GliB1.2 allele corresponds to the Gli-B1e, Gli-B1g and Gli-B1c variants. A new PCR allele with primers for marker GliB1.1 at the Gli-B1 locus in the GLI-B1-12 line (with the gliadin Gli-Blo block), which was generated from crossing of Bezosta 1 and the variety Levent, was detected. |
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