Histochemical and immunochemical studies on mammalian striated muscle fibres

Summary In 15 rabbit and 2 human distinct types of striated muscle, it was shown that all fibres classified as type I (red) by NADH-diaphorase and myofibrillar ATPase reaction exhibit intense fluorescence when treated with a FITC-labelled antibody against human pectoral actomyosin. Type II (white) fibres showing minimal fluorescence, an intermediate fibre type can be differentiated by all three methods. In the rabbit, all three fibre types are found, whereas, in the human muscles studied, only type I and intermediate type fibres were found.Abbreviations ATPase adenosintriphosphatase (EC 3.6.1.3) - anti-PAM anti-human-pectoralis actomyosin - FITC fluoresceine-isothiocyanate